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Comprehensive two-dimensional liquid chromatography – mass spectrometry for lipidomic analysis
Autoři: Ovčačíková Magdaléna | Cífková Eva | Lísa Miroslav | Holčapek Michal
Rok: 2014
Druh publikace: ostatní - přednáška nebo poster
Strana od-do: nestránkováno
Tituly:
Jazyk Název Abstrakt Klíčová slova
eng Comprehensive two-dimensional liquid chromatography – mass spectrometry for lipidomic analysis Lipids have numerous important functions in cells, tissues and body fluids, such as the formation of lipid bilayer, cell signaling, energy storage, etc. Lipidomics is a subset of metabolomics, which is aimed on the complex analysis of cellular lipid molecules isolated from cells, tissues or biological fluids and their biological roles with respect to health and disease states. Lipidomics contribute to the understanding how lipids work in a biological system (cellular physiology and pathology) [1]. One part of this work is focused on off-line two-dimensional liquid chromatography - mass spectrometry (2D-LC/MS) analysis of plasma samples, where the total lipid extract of plasma was separated into individual fractions of polar and nonpolar lipid classes. In the first dimension, 6 classes of polar lipids and 3 classes of nonpolar lipids were isolated by hydrophilic liquid chromatography (HILIC), where compounds are separated according to their polarity. Individual fractions were subsequently measured in the second dimension by reversed-phase high-performance liquid chromatography (RP-HPLC), where the polar lipids are separated according to the acyl chain length and the number of double bonds. Nonpolar lipid fractions are separated according to the value of an equivalent carbon number [2]. Another part of this work is focused on the development of on-line two-dimensional ultra high-performance liquid chromatography mass - spectrometry (2D-UHPLC/MS) method for the analysis of lipids. For this purpose, RP-LC mode is used in the first dimension, while HILIC is used in the second dimension of comprehensive 2D-LC/MS setup. Lipids were identified from extract of plasma sample using this comprehensive on-line 2D-UHPLC with ESI-MS in positive ion mode. Acknowledgement This work was supported by ERC CZ grant project LL1302 (Ministry of Education, Youth and Sports of the Czech Republic).

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