Publikace detail

Comparison of acetaminophen hepatotoxicity and nephrotoxicity in vitro

Autoři: Roušar Tomáš | Vrbová Martina | Kučera Otto | Roušarová Erika | Červinková Zuzana

Rok: 2014

Druh publikace: ostatní - článek ve sborníku

Název zdroje: Toxicology Letters

Název nakladatele: Elsevier Ireland Ltd

Místo vydání: Shannon

Strana od-do: S81

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Comparison of acetaminophen hepatotoxicity and nephrotoxicity in vitro	Acetaminophen (APAP) is a frequently used analgesic and antipyretic. The overdose can cause hepatotoxicity and nephrotoxicity. Acetaminophen toxicity has been considered as the most often cause of acute liver failure and that is why APAP hepatotoxicity has been studied in a number of in vitro studies. On the other hand, acetaminophen nephrotoxicity has been estimated only in some experiments. Therefore, the goal of our study was to compare hepatotoxic and nephrotoxic effect of acetaminophen in vitro. We used cultivated rat hepatocytes to characterize APAP hepatotoxicity. To elucidate the acetaminophen nephrotoxicity, we used human kidney cells (HK-2). Both cell types were incubated with acetaminophen (0-10 mM) for up to 24 h. We measured the LDH leakage, WST-1 to determine the changes in viability. Glutathione content and ROS production was measured for detection of oxidative stress.	Acetaminophen; mitochondria; toxicity; glutathione; ROS

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