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New lipidomics approach: High-throughput nontargeted lipidomic analysis using supercritical fluid chromatography/ion-mobility mass spectrometry
Autoři: Lísa Miroslav | Holčapek Michal
Rok: 2014
Druh publikace: ostatní do riv
Strana od-do: nestránkováno
Tituly:
Jazyk Název Abstrakt Klíčová slova
cze New lipidomics approach: High-throughput nontargeted lipidomic analysis using supercritical fluid chromatography/ion-mobility mass spectrometry Lipidomic analysis is challenging due to enormous complexity of lipid structures. Two well-established analytical approaches are commonly used in lipidomics, such as shotgun ESI-MS/MS and LC/MS, both having some advantages and limitations. Alternative high-throughput and nontargeted approach is developed in this work using supercritical fluid chromatography (SFC) followed by ESI and ion mobility-mass spectrometry (IM-MS) for the determination of lipid composition. Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the separation of 26 nonpolar and polar lipid classes within 6 minutes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification and quantitation of individual species. The quantitative analysis is performed using internal standards for each lipid class. IM is used as an additional separation dimension supporting the identification of individual lipids. Developed SFC/MS method is applied for the comprehensive analysis of lipid composition in real lipid samples. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic).
eng New lipidomics approach: High-throughput nontargeted lipidomic analysis using supercritical fluid chromatography/ion-mobility mass spectrometry Lipidomic analysis is challenging due to enormous complexity of lipid structures. Two well-established analytical approaches are commonly used in lipidomics, such as shotgun ESI-MS/MS and LC/MS, both having some advantages and limitations. Alternative high-throughput and nontargeted approach is developed in this work using supercritical fluid chromatography (SFC) followed by ESI and ion mobility-mass spectrometry (IM-MS) for the determination of lipid composition. Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the separation of 26 nonpolar and polar lipid classes within 6 minutes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification and quantitation of individual species. The quantitative analysis is performed using internal standards for each lipid class. IM is used as an additional separation dimension supporting the identification of individual lipids. Developed SFC/MS method is applied for the comprehensive analysis of lipid composition in real lipid samples. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic). lipidomics; nontargeted; lipidomic analysis; supercritical fluid chromatography; ion-mobility; mass spectrometry

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