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TESTING OF COLLAGENASE COVALENTLY LINKED TO CHITOSAN NANOFIBERS FOR BIOMEDICAL APPLICATIONS
Autoři: Slováková Marcela | Srbová Jana | Munzarová Marcela | Dvořáková Petra | Bílková Zuzana
Rok: 2015
Druh publikace: článek ve sborníku
Název zdroje: Nanocon 2015: Conference Proceedings
Název nakladatele: TANGER, spol. s r.o.
Místo vydání: Ostrava
Strana od-do: 1-6
Tituly:
Jazyk Název Abstrakt Klíčová slova
cze Testování chitosanových nanovláken s kolagenázou určených pro biomedicínské aplikace Testování chitosanových nanovláken s kolagenázou určených pro biomedicínské aplikace. chitosan;nanovlákna;kolagenáza;enzymová imobilizace;Michaelis-Menten
eng TESTING OF COLLAGENASE COVALENTLY LINKED TO CHITOSAN NANOFIBERS FOR BIOMEDICAL APPLICATIONS Nanofibers biofunctionalized by enzymes is materials of interest in the biomedical field due to their potential application in wound healing. This work describes the production and characterisation of electrospun chitosan nanofibers biofunctionalized by microbial collagenase. The morphology and microstructure of the chitosan nanofibers prepared by Nanospider technology were examined using a scanning electron microscopy (SEM). Amino groups of chitosan nanofiber were connected with the carboxylic acid groups of collagenase using EDAC and sulfo-NHS methodology. Collagenase enzymatic activity was evaluated by peptidic substrate (Pz-peptide, 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg) in order to estimate the ability of this biomaterial to be used as an enzymatic debriding wound dressing. Characterisation of collagenase chitosan nanofibers in term of apparent Michaelis-Menten constants (KM(app) and vmax) measurements revealed higher enzyme to substrate affinity in comparison with soluble form of collagenase. The resulting novel collagenase chitosan nanofibers showed excellent operational stability and long shelf life for at least 6 weeks. Therefore, the novel collagenase chitosan nanofibers are expected to be a potential scaffold for wound healing applications, as e.g. enzymatic debridement. chitosan nanofibers;collagenase;enzyme immobilization;Michaelis-Menten constant

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