Univerzita PardubiceFakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

„in-house“ labeling protocol for routine preparation of quantum dot-based conjugates Suited for electrochemical detection

Autoři: Dvořáková Veronika | Čadková Michaela | Datínská Vladimíra | Chalupniak Andrzej | Merkoci Arben | Klepárník Karel | Foret František | Bílková Zuzana | Korecká Lucie

Rok: 2015

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	„in-house“ labeling protocol for routine preparation of quantum dot-based conjugates Suited for electrochemical detection	Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with following optical and electrochemical detection. This approach is essentially based on carbodiimide chemistry however each single step of whole procedure is separated and controlled which makes this applied protocol oriented, highly efficient and it enables gain of pure antibody-conjugated quantum dots in ready-to-use condition.	quantum dots; conjugation protocol; IgG molecules