Publikace detail

Study of antitumor effect of selected V and Mo organometallic complexes in human leukemic T-cells

Autoři: Šebestová Lucie | Vinklárek Jaromír

Rok: 2015

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Study of antitumor effect of selected V and Mo organometallic complexes in human leukemic T-cells	This work describes cytotoxic effect of non-platinum compounds on the human T-leukemic cells with different p53 status (p53wild-type MOLT-4 and p53-deficient Jurkat cells). The cytotoxic and apoptosis-inducing effect of the vanadium complex [(η5-C5H5)2V(5-NH2-phen)]OTf (V1) and molybdenum complex [(η3-C3H5)Mo(CO)2(phen)Cl] (Mo1) were studied using flow cytometry, spectrophotometry and Western blotting. We found that the cytotoxic effect of both tested complexes is higher on the both examined cell lines than that of cis-platin. Furthermore we observed that the apoptotic process was induced by the activation of the caspase 9 via the inner apoptotic pathway. In case of the p53 wild-type MOLT-4 cells, the expression of the tumor-supressor gene p53 and its phosphorylated form at the serine 15 increased after both V1 and Mo1 treatment, similar to the effect of cis-platin.	vanadium; molybdenum

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