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IMPORTANCE OF PALMITOLEATE FOR THE TYPE 2 DIABETES PREVENTION

Autoři: Líbalová Martina | Laštovička Petr | Čermák Tomáš | Čegan Alexander

Rok: 2015

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	IMPORTANCE OF PALMITOLEATE FOR THE TYPE 2 DIABETES PREVENTION	Objectives: This study is focused on the research on effects of the palmitoleic acid in the individual lipid fractions. According to the resent researches the palmitoleic acid associates with diabetes mellitus type 2. The aim of this study is to understand the metabolic changes and to explain the role of the palmitoleic acid. Methods: Lipid extracts from deproteinated plasma samples were separated by preparative thin-layer chromatography into five fractions (phospholipids, diacylglycerols, free fatty acids, triglycerides and cholesterol esters). All fractions were isolated and after the addition of internal standard (cis-13,16,19-docosatrienoic acid, 10 µg / ml) hydrolyzed and converted to corresponding methyl esters of fatty acids. Thus prepared samples were analyzed using a gas chromatograph. Results: 27 anonymized plasma samples randomly selected type 2 diabetic patients were processed in this study. These samples of biological material were also used for verification of analytical method. Furthermore, there were used 20 healthy blood donors plasma samples as a control group. For purposes of the study, samples were categorized into three groups according to level of glycosidic hemoglobin (HbA1c). This indicator appears to be most reliable because it is affected by poor conditions (such as non fasting) prior to collection, and highlights the development of blood glucose in the last 4 to 6 weeks. Conclusions: Statistical analysis confirm a significant change in the concentration of palmitoleic acid in the free fatty acids fraction. The control group and diabetics were compared. The interesting results were collected from the vaccenic acid, its concentrations changed in the every fraction, except the diglycerid fraction. The vaccenic acid is a product of associate the palmitoleic acid.	diabetes mellitus, palmitoleate, gas chromatography, thin layer chromatography

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