Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

Zaměstnanci
|
Studenti
English

Publikace detail

THE ROLE OF CLEANING PROCEDURES IN ANALYSIS OF HAIR

Autoři: Krejčová Anna | Bendakovská Lenka | Návesník Jakub | Černohorský Tomáš

Rok: 2015

Druh publikace: ostatní - článek ve sborníku

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	THE ROLE OF CLEANING PROCEDURES IN ANALYSIS OF HAIR	As useful environmental biomonitors, hair and fur have been used to demonstrate the organism load with various persistent bioaccumulative or doping substances and heavy metals. Firmly built in the protein structure, endogenous substances can be released after the irreversible destruction of the hair structure. After a direct contact with the environment, exogenous chemicals trapped on hair surface can be removed by a washing process. Difficulties and unclearness are connected just with cleaning prior to sample preparation and analysis. Although previous studies recommended many washing procedures, only one unified IAEA procedure has existed (acetone and water). Especially washing steps bring potential risk of disruption of the hair internal structure and releasing of substances that are the subject of analytical interest. A suitable washing procedure useable before hair analysis was search for. The effect of single cleaning media (HNO3, acetone, Triton X-100, EDTA) was evaluated with the use of statistical methods for the DOE (a full factorial experiment, four factors at two levels). The optimization of cleaning conditions (concentration of washing media, time, and agitation support) was assessed using a reduced 2k-p factorial plan. 15 elements were followed including these which set up the border between exogenous (sodium, potassium) and endogenous (sulphur, copper) indicators. Significant differences between agents were found: each of them revealed different washing strength for specific elements (e.g. acetone released sulphur from the inner structure). Results brought a question if to compromise a washing step before multielemental analysis or if to use the procedure tailored to a single element.	horsehair, sample preparation, design of experiment, ICP-OES / MS