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Benfluron Induces Cell Cycle Arrest, Apoptosis and Activation of p53 Pathway in MOLT-4 Leukemic Cells

Autoři: Seifrtová Martina | Cochlarová Tereza | Havelek Radim | Řezáčová Martina

Rok: 2015

Druh publikace: článek v odborném periodiku

Název zdroje: Folia Biologica

Strana od-do: 147-155

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Benfluron indukuje zástavu buněčného cyklu, apoptózu a aktivaci cesty p53 u leukemických buněk MOLT-4	Práce popisuje efekt potenciálního chemoterapeutika benfluronu na leukemické buňky MOLT-4 . Cílem práce objasnit molekulární mechanismy působení benfluronu.	benfluron; apoptóza; buněčný cyklus; MOLT-4; p53
eng	Benfluron Induces Cell Cycle Arrest, Apoptosis and Activation of p53 Pathway in MOLT-4 Leukemic Cells	The aim of our study was to determine the effect of potential anti-tumour agent benfluron on human leukemic cells MOLT-4 and elucidate the molecular mechanisms of response of tumour cells to this chemotherapeutic agent. It has been shown that the mechanisms of action of benfluron are complex, but the molecular pathways of the cytostatic effect have remained unknown and the present study contributes to their elucidation. In this work, benfluron reduced viability of the treated cells and induced caspase-mediated apoptosis. The programmed cell death was associated with activation of caspases 8, 9 and 3/7. Moreover, exposure of cells to benfluron resulted in accumulation of the cells primarily in late S and G2/M phases. The changes in the levels of key proteins show that benfluron provoked activation of p53 and induced phosphorylation of p53 on serine 15 and serine 392. The application of benfluron led to phosphorylation of Chk1 on serine 345 and phosphorylation of Chk2 on threonine 68 in the treated cells. Higher doses of benfluron caused phosphorylation of ERK1/2 on threonine 202 and tyrosine 204, whereas JNK and p38 kinases were not activated. In conclusion, benfluron induces apoptosis, cell cycle arrest in late S and G2/M phases, and activates various signalling pathways of the DNA damage response.	benfluron; apoptosis; cell cycle; MOLT-4; p53

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