Univerzita PardubiceFakulta chemicko- technologická

Univerzita Pardubice

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English

Publikace detail

Comparison of toxic effects of acetaminophen, glutathione and an acetaminophen-metabolite in liver mitochondria

Autoři: Roušarová Erika | Vrbová Martina | Čapek Jan | Kučera Otto | Červinková Zuzana | Roušar Tomáš

Rok: 2015

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Comparison of toxic effects of acetaminophen, glutathione and an acetaminophen-metabolite in liver mitochondria	Acetaminophen belongs among mostly used antipyretics and analgesics. It is considered to be safe at therapeutic doses. However, after overdose, it can lead to hepatotoxicity followed by acute liver failure. The cause of cell damage has been found in its activation to an oxidized compound that is detoxified through glutathione. However, some studies showed that glutathione conjugates may exert also their toxic effects. Therefore, the aim of our study was to describe the effect of acetaminophen (APAP), its metabolite and glutathione in isolated liver mitochondria. We used rat, murine and also human isolated mitochondria for determination of reactive species and for characterization of cellular respiration. We used a fluorescence probe DCFDA to determine production of reactive species and respirometry to characterize the cellular respiration in presence of tested compounds. We found that mitochondrial function was inhibited in presence of newly synthetized APAP-related metabolite.