Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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English

Publikace detail

Prediction of retention data for fast gradient on 5-μm core-shell columns

Autoři: Hájek Tomáš | Růžičková Marie | Jandera Pavel

Rok: 2015

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Prediction of retention data for fast gradient on 5-μm core-shell columns	5 μm core-shell stationary phases are able to offer efficiencies that are at least comparable with fully-porous 3 μm stationary phases at the pressure of a 5 μm particle. Pressure reduction allows increase flow rate and perform fast separations. However, fast gradient employing short columns and gradient times in 5 min or even less under gradient conditions, impose stringent requirement on the accuracy of prediction, due to rapid changes in mobile phase composition and increased role of extra-column contributions and instrumental gradient dwell volume on the retention [1]. We studied possibilities of prediction of the gradient elution data for alkylbenzenes, phenolic acids and flavones on 5 um core-shell columns with different type of bonded stationary phases in fast 1 - 10 min gradients. The gradient elution data were predicted from four gradient models with the parameters of two two-parameter and two three-parameter isocratic retention equations. We compare the results with most frequently used commercial optimization software, DryLab, employs two initial scouting gradients, a short one and a long one, to find optimum gradient range. Furthermore, we investigated peak capacity and gradient number of theoretical plates. 5 μm core-shell columns were employed in the second dimension coupled with monolithic zwitterionic microcolumn [2] in the first dimension of comprehensive 2D-LC for separation of phenolic and flavonoid compounds.