Univerzita PardubiceFakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Effect of Gradient Steepness on the Kinetic Performance Limits and Peak Compression for Reversed-Phase Gradient Separations of Small Molecules

Autoři: Vaňková Nikola | De Vos Jelle | Tyteca Eva | Desmet Gert | Edge Tony | Česlová Lenka | Česla Petr | Eeltink Sebastiaan

Rok: 2015

Druh publikace: ostatní - článek ve sborníku

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Effect of Gradient Steepness on the Kinetic Performance Limits and Peak Compression for Reversed-Phase Gradient Separations of Small Molecules	Since the introduction of HPLC there has been a trend to decrease the particle size in order to increase the separation efficiency and to reduce the analysis time. In addition, stationary-phase design has evolved and core-shell particles have been introduced as alternative for fully-porous particles. An addition gain in efficiency can be realized when applying gradient-elution mode instead of isocratic mode. When a band migrates though the analytical column and a mobile phase is applied with increased solvent strength, the tail of the peak will move in the mobile phase that is slightly stronger compared to front of the same band. As a result, the tail of the peak tends to move faster and a peakfocusing effect is observed, which is referred to as gradient peak compression (G).