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Alterations in protein and amino acid metabolism in rats fed a branched-chain amino acid- or leucine-enriched diet during postprandial and postabsorptive states
Autoři: Holeček Milan | Šiman Pavel | Vodeničarovová Melita | Kanďár Roman
Rok: 2016
Druh publikace: článek v odborném periodiku
Název zdroje: Nutrition and Metabolism
Název nakladatele: Biomed Central, Ltd.
Místo vydání: London
Strana od-do: "12-1"-"12-12"
Tituly:
Jazyk Název Abstrakt Klíčová slova
cze Změna metabolismu proteinů a aminokyselin u potkanů krmených potravou obohacenou o aminokyseliny s rozvětveným řetězcem nebo leucinem během postprandiálního a postresorpčního stavu Článek pojednává o změně metabolismu proteinů a aminokyselin u potkanů krmených potravou obohacenou o aminokyseliny s rozvětveným řetězcem nebo leucinem během postprandiálního a postresorpčního stavu. Nutriční doplňky; glutamin; proteosyntéza; proteolýza; sval; hladovění
eng Alterations in protein and amino acid metabolism in rats fed a branched-chain amino acid- or leucine-enriched diet during postprandial and postabsorptive states Background: Many people believe in favourable effects of branched-chain amino acids (BCAAs; valine, leucine, and isoleucine), especially leucine, on muscle protein balance and consume BCAAs for many years. We determined the effects of the chronic intake of a BCAA-or leucine-enriched diet on protein and amino acid metabolism in fed and postabsorptive states. Methods: Rats were fed a standard diet, a diet with a high content of valine, leucine, and isoleucine (HVLID), or a high content of leucine (HLD) for 2 months. Half of the animals in each group were sacrificed in the fed state on the last day, and the other half were sacrificed after overnight fast. Protein synthesis was assessed using the flooding dose method (L-[3,4,5-3H] phenylalanine), proteolysis on the basis of chymotrypsin-like activity (CHTLA) of proteasome and cathepsin B and L activities. Results: Chronic intake of HVLID or HLD enhanced plasma levels of urea, alanine and glutamine. HVLID also increased levels of all three BCAA and branched-chain keto acids (BCKA), HLD increased leucine, ketoisocaproate and alanine aminotransferase and decreased valine, ketovaline, isoleucine, ketoisoleucine, and LDL cholesterol. Tissue weight and protein content were lower in extensor digitorum longus muscles in the HLD group and higher in kidneys in the HVLID and HLD groups. Muscle protein synthesis in postprandial state was higher in the HVLID group, and CHTLA was lower in muscles of the HVLID and HLD groups compared to controls. Overnight starvation enhanced alanine aminotransferase activity in muscles, and decreased protein synthesis in gastrocnemius (in HVLID group) and extensor digitorum longus (in HLD group) muscles more than in controls. Effect of HVLID and HLD on CHTLA in muscles in postabsorptive state was insignificant. Nutritional supplements; Glutamine; Protein synthesis; Proteolysis; Muscle; Starvation

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