Publikace detail

Anticancer potential of Amaryllidaceae alkaloids evaluated by screening with a panel of human cells, real-time cellular analysis and Ehrlich tumor-bearing mice

Rok: 2017

Druh publikace: článek v odborném periodiku

Název zdroje: Chemico-biological Interactions

Název nakladatele: Elsevier Ireland Ltd

Místo vydání: Shannon

Strana od-do: 121-132

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Protinádorový potenciál alkaloidů čeledi Amaryllidaceae testovaný na panelu lidských linií, pomocí analýzy buněk v reálném čase a na Ehrlichově nádoru myší	V této studii byl testován protinádorový potenciál 22 alkaloidů čeledi Amaryllidaceae na panelu lidských linií, pomocí analýzy buněk v reálném čase a na Ehrlichově nádoru myší.	Antiprolifeační aktivita; Amaryllidaceae; Alkaloidy; In vitro; In vivo
eng	Anticancer potential of Amaryllidaceae alkaloids evaluated by screening with a panel of human cells, real-time cellular analysis and Ehrlich tumor-bearing mice	In this study, twenty-two Amaryllidaceae alkaloids were screened for their anticancer potential. All isolates were evaluated for antiproliferative activities on a panel of 17 human cell types of different tissue origin using WST-1 assay. In addition, we determined the antiproliferative effect with a real-time cell analysis xCELLigence system. Thereafter, to evaluate the barely known in vivo anticancer potential of the most potent molecule haemanthamine, a preliminary study was performed using an Ehrlich tumor-bearing mice model. The results showed that haemanthamine, lycorine and haemanthidine exerted the highest antiproliferative activity. The mean growth percent (GP) value after a single-dose 10 mu M treatment was for haemanthamine 21%, for lycorine 21% and for haemanthidine 27% that of untreated control cells (100%). Furthermore, haemanthamine, lycorine and haemanthidine exhibited significant cytotoxicities against all the tested cell lines with individual IC50 values in the micromolar range. Dynamic real-time measures of impedance by xCELLigence indicated that these three compounds suppress cell proliferation after 10 h of treatment at a concentration of 10 mM or higher. Regrettably, in a follow-up in vivo antitumor activity study, haemanthamine showed no statistically significant reduction in the tumor size with no prolongation of survival time of Ehrlich tumor-bearing mice. Taken together, these results provide a new clue and guidance for exploiting Amaryllidaceae alkaloids as anticancer agents.	Antiproliferative activity; Amaryllidaceae; Alkaloids; In vitro; In vivo

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