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NANOPARTICLES AS THE KEY DETECTION COMPONENT OF SANDWICH-TYPE ELECTROCHEMICAL IMMUNOSENSORS FOR SIMULTANEOUS QUANTIFICATION OF OVARIAN CANCER BIOMARKERS

Rok: 2018

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	NANOPARTICLES AS THE KEY DETECTION COMPONENT OF SANDWICH-TYPE ELECTROCHEMICAL IMMUNOSENSORS FOR SIMULTANEOUS QUANTIFICATION OF OVARIAN CANCER BIOMARKERS	Despite medical progress, reliable test for accurate ovarian cancer diagnosis still does not exist. Therefore, cancer is recognized due to the lack of distinctive symptoms in the late stage and chances of survival are reduced. Simultaneous detection of multiple serum ovarian carcinoma biomarkers could significantly help to diagnostics. Moreover, detection of low levels of biomarkers is the critical parameter influencing the revelation of the early stage disease, which will allow more successful treatment. Electrochemical immunosensors employing highly selective affinity antigen-antibody recognition and combined with sensitive electrochemical detection are presented as potential candidates for multiple biomarkers detection with the choice of suitable labels of antibodies responsible for the resulting signal. The signal-generating tags are increasingly represented by nanomaterials (quantum dots nanocrystals, metal or silica nanoparticles, graphene, etc.) which are more than a suitable substitution for commonly exploited less stable enzymes. The appropriate nanomaterials in electrochemical immunosensors allow not only the amplification of the measured signal but also the possibility of simultaneous detection of multiple biologically important substances in one analysis . Three selected ovarian carcinoma biomarkers, namely human epididymis protein (HE4), cancer antigen 125 (CA 125) and alpha-fetoprotein (AFP) were analysed simultaneously by sandwich-type magneto-immunoassay. Specific antibodies against target biomarker are tagged with core type of quantum dots- (CdTe, PbS QDs) or gold nanoparticles-modified silica nanoparticles and applied for assembly of electrochemical immunosensor. The measurement of each probe was carried out by using square wave anodic/cathodic stripping voltammetry, and disposable screen-printed three-electrode sensors (SPCE) with in situ formed bismuth film.	ovarian cancer; simultaneous analysis; electrochemical detection;

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