Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Estimation of Redox Status in Kidney Cells Treated with Cadmium

Autoři: Handl Jiří | Čapek Jan | Majtnerová Pavlína | Roušar Tomáš

Rok: 2018

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Estimation of Redox Status in Kidney Cells Treated with Cadmium	Cadmium is a highly toxic heavy metal. The toxic effect of cadmium is most commonly detected in kidney. Acute exposure of the cells to cadmium can lead to apoptosis. Cadmium-induced cell death is linked with oxidative stress. Glutathione is the main intracellular nonprotein thiol with antioxidant properties in cells playing an essential role in protection against oxidative stress. Therefore, glutathione levels may be reduced in presence of cadmium-induced oxidative stress. Our aim was to characterize the redox changes in cells after cadmium treatment. A human proximal tubular cell line was used for studying nephrotoxicity in vitro. Proximal tubular cells were treated with CdCl2 at concentrations 0-200 µM for 2, 6 and 24 h. To evaluate the induction of oxidative stress after CdCl2 treatment, we measured intracellular reactive oxygen species (ROS) production and glutathione levels using intracellular probes and bimanes, respectively. ROS production increase was detected in cells treated with 100 and 200 µM CdCl2 after 2 and 6 h. After 24 h, no increase in ROS production was detected in 100 µM and 200 µM CdCl2 treated cells. After 24 h of treatment with 25 µM CdCl2, we found substantial enhancement of ROS production. Significant depletion of intracellular glutathione appeared in all incubation periods following the increase in ROS production. However, the finding of ROS production increase was not linked with glutathione depletion after 24 h of treatment with 25 µM CdCl2. We conclude that CdCl2 is a potent inducer of oxidative stress in tubular cells associated with ROS production and reduction of glutathione levels. The level of oxidative stress is depends on the dose and time of CdCl2 treatment.	Cadmium toxicity; Kidney Cells; Oxidative stress; Glutathione