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Electrochemical Analysis of Glycoprotein Samples Prepared on a Pneumatically-controlled Microfluidic Device
Autoři: Novotný Jakub | Ostatná Veronika | Foret František
Rok: 2019
Druh publikace: článek v odborném periodiku
Název zdroje: Electroanalysis
Strana od-do: 1994-2000
Tituly:
Jazyk Název Abstrakt Klíčová slova
cze Electrochemical Analysis of Glycoprotein Samples Prepared on a Pneumatically-controlled Microfluidic Device In this work, we designed and manufactured a microfluidic device for isolation and purification of glycoprotein samples. The conceived sample preparation device was fabricated in polycarbonate by micro-milling. The flow control and the fluid dosage into the micro-channels was solved by equipping the device with integrated pneumatic valves. The biochemical functionality was provided by beaded support modified by molecules with affinity to glycoproteins which was stacked inside the micro-channel reminiscent of packed affinity columns used in glycoprotein lectin assays. Unlabeled glycoproteins, namely fetuin, asialofetuin, and prostate-specific antigen, were voltammetrically analyzed using catalytic peak H at silver amalgam electrode.
eng Electrochemical Analysis of Glycoprotein Samples Prepared on a Pneumatically-controlled Microfluidic Device In this work, we designed and manufactured a microfluidic device for isolation and purification of glycoprotein samples. The conceived sample preparation device was fabricated in polycarbonate by micro-milling. The flow control and the fluid dosage into the micro-channels was solved by equipping the device with integrated pneumatic valves. The biochemical functionality was provided by beaded support modified by molecules with affinity to glycoproteins which was stacked inside the micro-channel reminiscent of packed affinity columns used in glycoprotein lectin assays. Unlabeled glycoproteins, namely fetuin, asialofetuin, and prostate-specific antigen, were voltammetrically analyzed using catalytic peak H at silver amalgam electrode. glycoprotein; microfluidics; square wave voltammetry

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