Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Analysis of Alachlor, Acetochlor and Metolachlor by Liquid Chromatography Coupled with Tandem Mass Spectrometry

Autoři: Kašpar Michal

Rok: 2021

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Analysis of Alachlor, Acetochlor and Metolachlor by Liquid Chromatography Coupled with Tandem Mass Spectrometry	Alachlor, acetochlor and metolachlor are herbicides used in the agricultural industry for control of broadleaf weeds. These substances are toxic to aquatic organisms. Analysis of these substances by liquid chromatography in reversed phase (RPLC) mode is difficult, because the compounds have very similar structure with the same retention on classical C18 columns. An alternative approach thus involves coupling of RPLC with MS / MS (tandem mass spectrometry) detection, which allows discrimination of these three herbicides in one sample. Analysis of these herbicides was performed by liquid chromatography in combination with electrospray ionization and triple quadrupole detection. A Kinetex C18 column (150×3 mm, 2.6 µm) was used for separation and mixture of methanol and water with ammonium acetate was used for gradient elution. The MS / MS parameters were optimized, the obtained fragmentation transitions (alachlor (270/239), acetochlor (270/225), metolachlor (284/253)) were used for MS / MS detection in the positive ion mode.