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A NEW METHOD FOR DETECTING TRAZODONE IN BIOLOGICAL FLUIDS

Autoři: Labzova Oleksandra | Hrdlička Vojtěch | Navrátil Tomáš | Matvieiev Oleksandr | Šelešovská Renáta

Rok: 2025

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	A NEW METHOD FOR DETECTING TRAZODONE IN BIOLOGICAL FLUIDS	A new method was developed and validated for the determination of antidepressant trazodone in human biological fluids. The method combines two techniques: hollow fiber-based liquid-phase microextraction (HF-LPME) and square wave voltammetry (SWV) using screen-printed boron-doped diamond electrode (SP-BDDE) [1]. The study established the optimal conditions with isoamyl benzoate as supported liquid membrane (SLM) immobilized in a polypropylene porous hollow fibre, 0.1 mol L−1 H2SO4 acceptor solution, Britton-Robinson buffer (pH = 10) as donor solution andextraction time of 35 minutes. After extraction, 10 μL of the acceptor solution was analyzed using SWV with the following optimized parameters: amplitude 55 mV, frequency 20 Hz, and potential step 4 mV. The combined HF-LPME-SWV method demonstrated excellent performance, with a linear range of 20-2000 nmol L−1. The limit of quantification (LOQ) was 42 nmol L−1 and the limit of detection (LOD) was 12 nmol L−1. Given that the therapeutic effect of Trazodone directly depends on maintaining itsphysiological levels, an objetvite method for controlling its dosage and absorption is critical [2,3]. The developed method was successfully applied to analyze human urine and blood plasma samples. Additionally, the study explored the electrooxidation mechanism of trazodone using mass-spectrometric analysis of electrogenerated products and reaction intermediates, proposing a two-step electro-oxidation mechanism.	Trazodone, Hollow fiber liquid-phase microextraction, Screen-printed boron-doped diamond electrode, Square wave voltammetry

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