Univerzita PardubiceFakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

COMPARISON OF 2D AND 3D MODELS DERIVED FROM GASTRIC CANCER N87 CELL LINE

Autoři: Vlček Antonín | Čapek Jan | Roušar Tomáš

Rok: 2025

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	COMPARISON OF 2D AND 3D MODELS DERIVED FROM GASTRIC CANCER N87 CELL LINE	Gastric cancer is among the leading cancer types in terms of incidence and mortality in many countries around the world with almost 1 million newly diagnosed cases per year. Cell culture models play an important role in understanding the pathophysiology of gastric cancer cells. At present, two‑dimensional (2D) cell culture is important, but advanced and more sophisticated three‑dimensional (3D) approaches have been replacing it in recent years. Main advantage of 3D cell culture is to mimic better the complex nature of gastric cancer for in vitro disease modelling. To understand better the differences between 2D and 3D cell culture models, we cultivated N87 gastric cancer cells as 2D monolayers and 3D spheroids. To characterize the cells, we performed several immunocytochemistry assays for detection of cytokeratin 18, carcinoembryonic antigen and selected mucin molecules. We also incubated N87 cells with chemotherapeutic agents, such as 5-fluoruracil (100 and 2500 µM) and cisplatin (50 µM) for up to 72 h. Glutathione levels, dehydrogenase activity and ATP content were chosen as indicators of cellular damage. In conclusion, using immunocytochemistry approaches, we detected epithelial and tumor nature of N87 cells together with their capability to express specific mucin molecules. The exposure of N87 cells to 5-fluorouracil and cisplatin induced cell impairment which was characterized by decreased levels of glutathione, reduction of dehydrogenase activity and ATP depletion in comparison to untreated cells. Cellular damage was potentiated in timeand dose‑dependent manner.	gastric cancer; N87 cell line; cytotoxicity, immunocytochemistry