Vývoj bioafinitních reaktorů pro detekci a analýzu epitopů s imunogenním nebo alergogenním potenciálem pomocí mikročipových analyzátorů
Poskytovatel: Grantová agentura ČR
Program: Standardní projekty
Období realizace: 01.01.05 - 31.12.07
Pracoviště:
Fakulta chemicko-technologická - Katedra biologických a biochemických věd
Hlavní řešitel: Bílková Zuzana
Popis:
The main goal of this project is to develop the microaffinproteomic systems for high-throughput microfluidic instrumentation. The bioaffinity, immunoaffinity and enzyme reactors will be applied for epitope screening of autoantigens or DNA molecules. New techniques of affinity proteomics as micro-immobilization chemistry, microproteolytic reactors, micro system for epitope-antibody and oligonucleotid-antibody interaction will be utilized. Proteolytic enzymes, IgG and other affinity ligands will be immobilized on magnetic micro- and nanospheres developed for microchip application. The immunogenic potential of peptides and oligonucleotides will be determined by the immunosorbent system with orientedly immobilized specific IgG. We will develop the complex of enzymatic micro-on-chip-reactors for total or limited digestion and gentle and specific modification of target peptide structure in order to evaluate the quality of affinity recognition. In such a way we can predict the main immunogenic structure of cryptic or sequestered epitopes, which can be common for antigens with autoimmunogenic potential.
The main goal of this project is to develop the microaffinproteomic systems for high-throughput microfluidic instrumentation. The bioaffinity, immunoaffinity and enzyme reactors will be applied for epitope screening of autoantigens or DNA molecules. New techniques of affinity proteomics as micro-immobilization chemistry, microproteolytic reactors, micro system for epitope-antibody and oligonucleotid-antibody interaction will be utilized. Proteolytic enzymes, IgG and other affinity ligands will be immobilized on magnetic micro- and nanospheres developed for microchip application. The immunogenic potential of peptides and oligonucleotides will be determined by the immunosorbent system with orientedly immobilized specific IgG. We will develop the complex of enzymatic micro-on-chip-reactors for total or limited digestion and gentle and specific modification of target peptide structure in order to evaluate the quality of affinity recognition. In such a way we can predict the main immunogenic structure of cryptic or sequestered epitopes, which can be common for antigens with autoimmunogenic potential.
