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Simultaneous Measurement of Malondialdehyde, Formaldehyde, Acetaldehyde, and Propionaldehyde to Monitor the Oxidative Stress in Men

Autoři: Kanďár Roman | Žáková Pavla | Čegan Alexander | Skalický Jiří

Rok: 2003

Druh publikace: ostatní - přednáška nebo poster

Název zdroje: Clinical Chemistry and Laboratory Medicine Special Supplement

Název nakladatele: Walter de Gruyter GmbH & Co. KG

Místo vydání: Berlin

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Jazyk	Název	Abstrakt	Klíčová slova
cze	Simultánní stanovení malondialdehydu, formaldehydu, acetaldehydu a propionaldehydu pro monitorování oxidačního stresu u lidí		malondialdehyde, formaldehyde, acetaldehyde, propionaldehyde, oxidative stress, HPLC, lipid peroxidation
eng	Simultaneous Measurement of Malondialdehyde, Formaldehyde, Acetaldehyde, and Propionaldehyde to Monitor the Oxidative Stress in Men	Reactive oxygen species are capable to initiate a radical chain reaction known as lipid peroxidation. Malondialdehyde and several other aldehydes and ketones are the degradation products of peroxidized lipids. Aim: To develop and to evaluate a method for the determination of malondialdehyde (MDA), formaldehyde (FDA), acetaldehyde (ADA), and propionaldehyde (PDA) simultaneously in plasma by HPLC with UV-VIS detection. Plasma samples were obtained from 10 blood donors (5 women and 5 men, age: 24 - 34 years). To precipitate proteins from the plasma was used 10% trichloroacetic acid. The supernatant was derivatized with 2,4-dinitrophenylhydrazine (DNPH), and after incubation the pyrazole and hydrazine derivates were extracted with pentane (threetimes). After evaporation to dryness at 37 °C under nitrogen, the dry residue was dissolved in 100 microL acetonitrile and 50 microL was injected onto a Biospher SI 120 PSI C18 (5 microm) column. By using controller, a gradient program was used to separate the aldehyde-DNPHs from DNPH and other interfering compounds. The flow rate was 1.0 mL/min. MDA-DNPH was detected at 310 nm; FDA-DNPH, ADA-DNPH, and PDA-DNPH at 360 nm. The analytical performance of the method is satisfactory, both inta-assay and inter-assay were with coefficient of variation below 10% for each of aldehydes. Conclusion: We have developed and evaluated a HPLC method for the determination of aldehydes, products of lipid peroxidation. This work supported by grants GACR 203/02/0023, MSMT 253 100 002.	malondialdehyde, formaldehyde, acetaldehyde, propionaldehyde, oxidative stress, HPLC, lipid peroxidation

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