Publikace detail

Glucose Biosensor with RhO2 and Glucose Dehydrogenase

Autoři: Polan Vojtěch | Soukup Jan | Vytřas Karel

Rok: 2009

Druh publikace: článek ve sborníku

Název zdroje: YISAC 2009 - Young Investigators Seminar on Analytical Chemistry

Název nakladatele: Karl-Franzens-Universitaet Graz

Místo vydání: Graz

Strana od-do: 32-35

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze
eng	Glucose Biosensor with RhO2 and Glucose Dehydrogenase	Several types of immobilization methods were tested with glucose oxidase, including entrapment in Nafion, cross-linking with glutaraldehyde, immobilization with cellulose acetate and electropolymerization of pyrrol or m-phenylenediamine. Subsequently, a glucose dehydrogenase enzyme together with cofactor NAD+ were immobilized using the best method, in terms of enzyme activity retaining, response time, sensitivity and dynamic range of concentrations. As the best method was evaluated immobilization of enzyme via electropolymerization with m-phenylenediamine. This way prepared enzyme biosensors has been optimized and tested in model samples of glucose. Biosensor had linear range 500 ? 5000 mg.L-1 of glucose with a detection limit of 210 mg.L-1 (evaluated as 3 sigma) and response time 39 s.	Biosensor;Glucose

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