Publikace detail

IMERs and IMARs: effective tool for isolation and study of highly heterogeneous glycoproteins

Autoři: Korecká Lucie | Ježová Jana | Slováková Marcela | Ouzká Šárka | Bílková Zuzana

Rok: 2004

Druh publikace: ostatní - přednáška nebo poster

Název zdroje: 5th International conference on the Scientific and Clinical Applications of Magnetic Carriers

Název nakladatele: neuveden

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Jazyk	Název	Abstrakt	Klíčová slova
cze	IMER aIMAR: efektivní nástroj pro izolaci a studium vysoce heterogenních glykoproteinů.	IMER aIMAR: efektivní nástroj pro izolaci a studium vysoce heterogenních glykoproteinů.	IMER; IMAR; magnetické partikule
eng	IMERs and IMARs: effective tool for isolation and study of highly heterogeneous glycoproteins	The newly developed magnetic micro- and nanoparticles with defined hydrophobicity and porosity (see table) were used for preparation of immobilized magnetic enzyme reactors (IMERs) and immobilized magnetic affinity reactors (IMARs). Magnetic particles with controlled tendency to aggregate and controlled level of nonspecific sorption enable highly efficient and gentle separation during immobilization, high specificity and reproducibility of catalyzed reactions without risk of contamination resulting mixture, elimination of the undesirable dilution and losses of the analyte. Nanometer sized magnetic carriers can be focused in a channel of magnetically active microfluidic analytical device. type of particles material size (ěm) function group developed by concentration immobilized ligand hydrophilic, nonporous alginate coated ferrite microparticles 5-10 -COOH IMCH AS CR 0.5% trypsin hydrophilic, nonporous cellulose magnetite microparticles 6-8 -OH IMCH AS CR 1.2% trypsin, laccase hydrophilic, macroporous bead cellulose 80-100 -OH IMCH AS CR powder papain , protein A hydrophobic, nonporous polyNIPAM 0.29 -COOH Ademtech, France 5% lectins hydrophobic, nonporous polySTYREN 0.86 -NH2 Uptima, France 4% trypsin hydrophobic, nonporous poly(HEMA-co-GMA) (50/50wt) 3.70 -OH IMCH AS CR powder trypsin hydrophobic, nonporous poly(HEMA-co-EDMA-co30%GMA) 3.70 -OH IMCH AS CR powder trypsin hydrophobic, nonporous polyNIPAM, 0.875 -COOH Ademtech, France 2% lectins Table. Specification of used magnetic carriers and its applications The factors as nature of carrier (size, porosity, hydrophilic or hydrophobic character, reactive groups on the surface, behavior of particles in magnetic field, paramagnetism, minimal non-specific adsorption of proteins, peptides) and immobilization procedure (time, temperature, intensity of mixing, environmental pH, rate of enzyme and carrier, necessity of ions, activators, surfactants) were controlled. As important factors ensuring pro	IMER; IMAR; magnetic particles

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