Publikace detail

Evaluation of glutathione metabolism in cultured rat hepatocytes during acetaminophen injury.

Rok: 2007

Druh publikace: článek ve sborníku

Název zdroje: Free Radical Research

Název nakladatele: Informa Healthcare UK

Místo vydání: London

Strana od-do: 51-52

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Monitorování metabolismu glutathionu v kultivovaných hepatocytech během toxického působení acetaminofenu	Monitorování metabolismu glutathionu v kultivovaných hepatocytech během toxického působení acetaminofenu
eng	Evaluation of glutathione metabolism in cultured rat hepatocytes during acetaminophen injury.	Acetaminophen (AAP) is a safe analgesic and antipyretic drug when used at therapeutic doses. On the other hand, the AAP overdose is one of the most frequent causes of drug-induced acute liver failure. Albeit the mechanisms of AAP injury have been extensively studied recently, some of them remain to resolve. Glutathione is an essential compound acting in detoxification of AAP because it binds to the active metabolite, N-acetyl-p-benzoquinone imine (NAPQI). After depletion of GSH levels, NAPQI binds to number of cellular proteins. The aim of our study was to evaluate the changes in glutathione metabolism, GSH and GSSG levels and glutathione reductase (GR) activity, during AAP injury. Especially, we focused on time course of changes with respect to increasing AAP doses. Hepatocytes were isolated from male Wistar rats by collagenase perfusion and cultivated on collagen-coated 24-well plates. Our results proved that the activity of GR is evidently decreased in relation to AAP concentration and time of incubation (after 24 h incubation with AAP 5 mM, 10 mM and 20 mM, respectively ? activity of GR compared to controls: 60+/-6%, 36+/-5% and 21+/-7%, respectively).	Acetaminophen; oxidative stress; hepatocyte

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