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Occurrence of potential toxigenic fungi in foodstuffs

Autoři: Vytřasová Jarmila | Brožková Iveta | Červenka Libor | Kudrnová Linda | Přibáňová Petra

Rok: 2005

Druh publikace: ostatní - přednáška nebo poster

Název zdroje: Czech Mycology

Název nakladatele: Česká vědecká společnost pro mykologii

Místo vydání: Praha

Strana od-do: 320

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Výskyt potenciálně toxinogenních plísní v potravinách	Výskyt potenciálně toxinogenních plísní v potravinách	Cladosporium sp.; Aspergillus niger; Penicillium; Acremonium; výskyt plísní; PCR
eng	Occurrence of potential toxigenic fungi in foodstuffs	The occurrence of fungi spores, especially those of potential toxigenic ones was monitored in different cereals and bakery products. Consumption of such a food could affect the human health due to presumptive mycotoxin production. The air is significant reservoir of fungal spores and may be responsible for their transmission to foods being contaminated during the processing, transporting and storing. Although cleaning procedure with addition of common disinfectant could successfully avoid bacterial contamination, fungal spores resisted. Hence food products are supplemented with chemical substances which could suppress the growth of fungi. The objective of this study was to isolate and identify fungi from bakery products and cereals. When potential toxigenic fungi belonging to the genus of Aspergillus and Fusarium were found, PCR technique was optimized and applied to detect their genes coding aflatoxin B1 and trichothecens productions. Primers specifically targeted to apa-2, ver-1 (Aspergillus sp.) and Tri-5 (Fusarium sp.) were used. Different chemical substances were also evaluated for their fungicidal properties. Cladosporium sp. and Aspergillus niger were the most frequently isolated species following by members of genera Penicillium and Acremonia. A. flavus and variety of xerophilic fungi (especially Eurotium sp.) were also isolated. From all conservation substances tested, both propionic acid and potassium sorbate found to be the most effective against the growth of isolated fungi. The pH dependence of antifungal properties was observed. Results indicate that occurrence of potential toxigenic fungi in bakery products represents an issue which should be minutely observed.	Cladosporium sp.; Aspergillus niger; Penicillium; Acremonium; occurrence of fungi; PCR

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