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BIOACTIVE MAGNETIC MICROREACTORS FOR STRUCTURAL MODIFICATIONS OF TAU PROTEIN MOLECULES USABLE IN THE STUDY OF PHOSPHORYLATION PROCESS AND DIAGNOSTIS OF ALZHEIMER´S DISEASE

Rok: 2011

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	BIOACTIVE MAGNETIC MICROREACTORS FOR STRUCTURAL MODIFICATIONS OF TAU PROTEIN MOLECULES USABLE IN THE STUDY OF PHOSPHORYLATION PROCESS AND DIAGNOSTIS OF ALZHEIMER´S DISEASE	Tau protein is a highly soluble microtubule-associated protein (MAP) mostly found in neurons compared to non-neuronal cells in humans. When misfolded this otherwise highly soluble protein can form an extremely insoluble aggregates that contribute to a number of neurodegenerative diseases. Hyperphosphorylation of the Tau protein can result in the self-assembly of tangles of paired helical filaments and straight filaments, which are involved in the pathogenesis of Alzheimer's disease (AD) and other tauopathies. Whereas recombinant Tau proteins are available only in unphosphorylated form, we ment prepare hyperphosphorylated Tau protein molecules, which could be used for the study of phosphorylation process and its pathological impact also. Efficient fragmentation of protein molecules before MS analysis improves identification of protein sequence and possible identification of phosphorylation-sites. We used proteolytic enzyme Clostripain, which cleaves proteins on the carboxyl peptide bond of arginin.	Tau protein;magnetic particles;Clostripain;GSK-3β;MAP kinase

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