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Chitosan nanofibre membranes for controlled release of proteolytic enzymes.

Autoři: Slováková Marcela | Kučerová Jana | Žárská Monika | Stránská Denisa | Bílková Zuzana

Rok: 2012

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	Chitosan nanofibre membranes for controlled release of proteolytic enzymes.	Our lab study covalent immobilizations of different proteases to solid carriers, and the aim of this work is to immobilize chymotrypsin on biopolymer nanofibers with sufficient enzyme activity, to investigate the effect of immobilization method, temperature, and pH on the preparation, its stability as possible drug delivery system in cutaneous dressings for wound healing. Critical steps in field of bioapplications are choices of polymer, nanofibre membrane technology with or without functional groups implementation, enzyme and its binding method (covalent bond, encapsulation, sorption), possible with non-toxic chemicals in processes used. Moreover, final product must meet aseptic conditions and sufficient storage stability for both membrane and enzyme. Goal of our work was to fabricate chitosan nanofibers and to biofunctionalize them by proteolytic enzymes. Chitosan nanofibers were prepared by needle-free high-voltage electrospinning process, the technology of NanospiderTM, of acidic chitosan solution and the nanofibrous layer was subsequently crosslinked by heating. Due to fact that chitosan membrane is in vivo degraded in time, covalently conjugated enzymes can be possibly released into surroundings. If proteolytic enzymes i.e. trypsin, chymotrypsin, lysozyme are used, it accelerates wound healing in two ways, first in degradation of necrotic tissue and second in decrease of exudate production. The proteolytic activity was quantified by low-molecular substrates, which was followed by yellow color production and absorbance at 405 nm measuring. Our results demonstrated that enzyme-immobilized nanofibers could well serve in wound healing applications. The authors wish to acknowledge the Ministry of Industry and Trade No. MPO TIP FR-TI1/0436 for financial support.	nanofibre membrane, proteolytic enzymes, chitosan, wound healing

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