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Transport of Biochemically Important Ions and Compounds across Biomimetic Membranes

Rok: 2014

Druh publikace: ostatní - článek ve sborníku

Název zdroje: 47th Heyrovsky discussion on electrochemistry of organic and bioactive compounds

Název nakladatele: Ústav fyzikální chemie Jaroslava Heyrovského AV ČR

Místo vydání: Praha

Strana od-do: 48

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Transport of Biochemically Important Ions and Compounds across Biomimetic Membranes	A large number of biologically important ions and compounds are coming into contact with plants, animals or men. Such compounds can be transported into these organisms, more precisely, into their cells, and after entering cells, into their subcellular structures (e.g., from cytosol to vacuole). Simultaneously, they are transported into different parts of organism (e.g., from plant roots to leaves). More precisely, any species taking part in metabolic processes must be first transported across the biological membranes. The latest results in elucidation transport processes of charged species across the biomimetic membranes will be presented in this contribution. The attention has been paid to the transport of hazardous metals (e.g., Cu, Cd) in free form as well as in form of their complexes (e.g., with low molecular weight organic acids, phytochelatins). Three different ways of preparation of biomimetic membranes, composed of simple phospholipids (PLs) (e.g., lecithin) have been realized: liposomes, self-assembling PL membranes (PLMs) on the agar surface [1], and PLMs in pores of polycarbonate substrate [2-5]. The biomimetic membranes composed of lecithin and cholesterol have been studied too [6]. Firstly, the real transporters were replaced by fat-soluble polypeptides [5]. Later, the transport processes have been studied using real membranes (protoplasts isolated from leaves of tobacco, potato, and barley). Finally, the real membranes mixed with model PLMs have been investigated. The PLMs and the transporting processes were characterized by electrochemical methods (electrochemical impedance spectroscopy (EIS), voltammetry, ion selective electrodes) as well as by non-electrochemical methods (optical microscopy, AFM, electrospray ionization mass spectrometry (ESI-MS) [5]).

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