Publikace detail

Hypercrosslinking modification of organic polymer-based monoliths

Autoři: Urban Jiří | Škeříková Veronika | Janků Simona | Hoznauerová Barbora

Rok: 2014

Druh publikace: článek ve sborníku

Název zdroje: 13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-3)

Název nakladatele: Royal Flemish Chemical Society (KVCV)

Místo vydání: Antwerp

Strana od-do: 71-72

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
cze	Modifikace povrchu organických polymerů vysokým zesítěním	Hyperkroslinkování umožňuje připravit organické polymerní kapilární kolony vhodné pro rychlou a účinnou separaci malých molekul za izokratických podmínek. Účinnost kolony koresponduje s rozsahem zesítění a může být ovlivňována polaritou rozpouštědla, reaktivitou a polaritou alkylačního katalyzátoru a teplotou a časem modifikační reakce.	Vysoké zesítění; organické monolity
eng	Hypercrosslinking modification of organic polymer-based monoliths	Hypercrosslinking post-polymerization modification allows preparation of organic polymer capillary monolithic columns suitable for fast and efficient separations of small molecules in isocratic liquid chromatography. Column efficiency corresponds to the extent of hypercrosslinking and can be tuned by several variables including polarity of the swelling solvent, reactivity and concentration of alkylation catalyst, and time and temperature of modification reaction. Three dihalogenic solvents differing in polarity (1,2-dichloroethane, 1,4-dichlorobutane, and 1,6-dichlorohexane) with three Friedel-Crafts alkylation catalysts varying in reactivity (AlCl3, FeCl3, and SnCl4) have been used to prepare hypercrosslinked poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) columns. After optimization of hypercrosslinking conditions including reaction time, temperature, and catalyst concentration, we prepared a column with efficiency exceeding 80 000 theoretical plates/m for benzene. Optimized hypercrosslinked columns enable fast separations at higher flow rates without the loss of efficiency as demonstrated by fast isocratic separation of low molecular compounds, such as barbiturates. We have also modified the surface of hypercrosslinked monoliths by zwitterionic monomer to prepare a column allowing a separation of polar compounds in hydrophilic interaction chromatography. The prepared column did not lose its separation power even after more than 10 000 injections which confirmed very high stability of the modification reaction. Finally, the optimized capillary monolithic column has been applied in comprehensive two-dimensional chromatography of polar phenolic acids and flavones.	Hypercroslinking; organic monoliths

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