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Development of Microfluidic Interface for On-line Two-dimensional Liquid Chromatography-Capillary Electrophoresis

Autoři: Česla Petr | Křenková Jana | Váňová Jana | Vaňková Nikola | Fischer Jan

Rok: 2014

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	Development of Microfluidic Interface for On-line Two-dimensional Liquid Chromatography-Capillary Electrophoresis	The two-dimensional liquid phase separations combine different separation modes or different techniques in both dimensions with low correlation resulting in highly orthogonal systems. On-line combination of liquid chromatography and capillary electrophoresis is not a trivial task as it requires compatibility of mobile phases with background electrolytes, matching column and capillary dimensions and proper electrical connection of the electrophoretic system. Moreover, the interface for coupling of both techniques should not significantly contribute to the band broadening and thus should not depreciate the resolution gained in the first separation dimension. In present work, some potential problems connected with the interfacing of LC and CE in an on-line system were investigated. A microfluidic interface for on-line coupling of both separation techniques was designed and fabricated using photolithographic technique. The interface design was numerically evaluated taking into account hydrodynamic flow, electrostatic and mass transfer using finite element approach, allowing detailed study of the fraction transfer process. The compatibility of mobile phases used in reversed-phase and hydrophilic interaction chromatography was tested with the volatile background electrolytes, which allow possible connection of second dimension capillary zone electrophoresis with mass spectrometry. The orthogonality of both techniques was verified by examining each technique independently for standard mixture of oligosaccharides. The phase selection and fraction modulation approach is illustrated by practical examples of on-line separation of biologically important glycans.

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