Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Mass spectrometry Based Lipidomics: State-of-Art and New Trends

Autoři: Holčapek Michal | Lísa Miroslav | Cífková Eva | Jirásko Robert

Rok: 2015

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Mass spectrometry Based Lipidomics: State-of-Art and New Trends	Shotgun ESI-MS/MS quantitative approaches are based on the direct infusion of total lipid extracts containing suitable internal standards per each quantified lipid class. The ion suppression effects could reduce the sensitivity for determination of trace lipids, but suppression effects can be compensated by internal standards and the quantitative data are reliable, as demonstrated in numerous large-scale clinical studies. Matrix-assisted laser desorption/ionization (MALDI)-MS is typically used without a chromatographic separation, but the limitation of MALDI is lower signal stability and reproducibility depending on the matrix used and the sample preparation. The limitation of non-separation approaches can be overcome by LC/MS techniques, which can be used the determination of various types of isobaric lipids, such as regioisomers, double bond positional isomers, cis/trans geometrical isomers and finally enantiomers. We will present examples of individual separation modes suitable for distinguishing of particular type of isomerism, such as reversed-phase, silver-ion or chiral chromatography used either in conventional HPLC mode or preferably under UHPLC conditions using sub-2 μm particles and high back pressures or alternatively core-shell sub-3 μm particles. Recently, the potential of ultrahigh-performance supercritical fluid chromatography (UHPSFC) has been realized with a great separation efficiency and very short analysis times compared to LC/MS, as will be illustrated on the example of 6 min method used for the quantitation of up to 30 lipid classes. Ion mobility used in configuration with mass spectrometry can bring supplementary separation of lipids from background ions and separation of lipid classes and species inside classes. Lipid species with the highest impact on the differentiation of tumor and normal tissues are identified.