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Lipidomic analysis of complex biological samples usin continuous comprehensive 2D LC/ESI-MS
Autoři: Ovčačíková Magdaléna | Lísa Miroslav | Cífková Eva | Hájek Tomáš | Holčapek Michal
Rok: 2015
Druh publikace: ostatní - přednáška nebo poster
Strana od-do: nestránkováno
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Jazyk Název Abstrakt Klíčová slova
eng Lipidomic analysis of complex biological samples usin continuous comprehensive 2D LC/ESI-MS Lipids have numerous important functions, such as the formation of lipid bilayer, cell signaling and energy storage. Their interaction with surrounding cells has a crucial role in living organism. Lipidomics is a subset of metabolomics, which is aimed on the complex analysis of cellular lipid molecules isolated from cells, tissues or biological fluids and their biological roles with respect to health and disease states. One-dimensional separation may not be sufficient for the analysis of complex lipidomic mixtures, therefore two-dimensional liquid chromatography–electrospray ionization mass spectrometry (2D LC/ESI-MS) method can be used to increase the number of compounds separated and quantitated in a single run. This work is focused on the development of continuous comprehensive 2D LC/ESI-MS for the lipidomic characterization of polar and nonpolar lipid classes in different biological samples. Reversed-phase liquid chromatography (RP-LC) mode used in the first dimension allows to obtain the best separation of the maximum number of lipid species according to fatty acyl chain length and the number of double bonds (DB). The fast hydrophilic interaction liquid chromatography (HILIC) method used in the second dimension allows the separation of overlapped lipids in the first dimension on the basis of their different polarities. Advantages of our new continuous comprehensive 2D LC/ESI-MS method are the shorter analysis time and easier automation, but the drawback is the lower number of identified lipids compared to fully optimized system.

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