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Selectivity od Separation of Natural Antioxidants in Gradient Reversed-phase Liquid Chromatography

Autoři: Palmieri Michela | Česla Petr | Česlová Lenka

Rok: 2015

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	Selectivity od Separation of Natural Antioxidants in Gradient Reversed-phase Liquid Chromatography	Polyphenols are a large family of compounds, including phenolic acids, flavonoids and tannins, widely distributed in plants and related food products. Many of them are natural antioxidants, which have beneficial effects on human health, by decreasing the level of free radicals in the organism; other polyphenols are toxic, harmful or even may have carcinogenic and mutagenic effects. These compounds also influence taste, flavor, color and durability of beer, wine, tea, fruit juice and other beverages. The polyphenols can be easily analyzed using liquid chromatography in reversed-phase mode; however, the optimization of separation selectivity is usually required especially for structurally similar or isomeric compounds. The purpose of the present work was to develop a systematic approach to the selection of columns and mobile phases suitable for separation of natural phenolic antioxidants, based on the correlations between the gradient retention times and molecular structure-descriptors of representative phenolic acid and flavonoid standards. For this aim, multiple linear regression, cluster analysis and window–diagram optimization strategies are combined. The gradient retention data for a set of phenolic compounds obtained using six different columns were used to establish the retention model. Predicted retention factors were then applied for evaluation of selectivity using linear free energy relationship concept, which enables comparison of selectivity of separation among different stationary phases used. Finally, the best predicted conditions were verified by analysis of natural antioxidants in real matrices.	polyphenols, retention behaviour, reversed-phase liquid chromatography

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