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Peak Capacity in Two-dimensional Separations Combining Liquid Chromatography with Capillary Electrophoresis

Rok: 2015

Druh publikace: ostatní - článek ve sborníku

Název zdroje: 22nd International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2015): book of abstracts

Název nakladatele: Finish Society for Chromatography and Mass Spectrometry

Místo vydání: Helsinky

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Peak Capacity in Two-dimensional Separations Combining Liquid Chromatography with Capillary Electrophoresis	The main purpose for application of two-dimensional separations is the increased peak capacity comparing to the unidimensional systems, which is especially needed for analysis of samples of biological origin. The highest increase of peak capacity with respect to the uni-dimensional separation is achieved for orthogonal separation mechanisms, i.e. for the combination of liquid chromatography with capillary zone electrophoresis. In present work, we have investigated the effects of separation conditions on peak capacity and peak production rate of two-dimensional separation utilizing combination of hydrophilic interaction liquid chromatography with capillary zone electrophoresis for separation of maltooligosaccharides and glycans. We have applied several retention models describing the partitioning of the analytes between the bulk mobile phase and adsorbed water-rich layer, polar and ionic interactions with various stationary phases including silica gel, aminopropyl-, carbamoyl- and zwitterionic sulfobetain-bonded silica gel. The fast electrophoretic separations utilizing conditions compatible with mass spectrometric detection (volatile background electrolytes and short separation capillaries) were used in the second-dimension separations. Finally, the theoretically obtainable peak capacities determined using retention/migration models for developed two-dimensional system were compared and discussed with respect to the undersampling effect observed due to the application of microfluidic flow-gating interface connecting both separation techniques.

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