Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

CLEANING PROCEDURES AND RELEVANCE OF HAIR ANALYSIS

Autoři: Krejčová Anna

Rok: 2016

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	CLEANING PROCEDURES AND RELEVANCE OF HAIR ANALYSIS	As a helpful environmental bioindicator, hair (fur) can demonstrate the organism load with various persistent bioaccumulative or doping substances and heavy metals. If stably built in the protein structure, these substances can be released after the irreversible destruction of the hair. Chemicals originating from the environment trapped on hair surface need to be removed by a washing process prior to analysis. Difficulties, unclearness and irreproducibility in results often come from a cleaning step prior to sample preparation. Although previous studies presented and recommended many washing procedures, only one unified IAEA procedure (using acetone and water) has existed. Especially washing steps bring potential risk of disruption of the hair internal structure and releasing of substances that are the subject of analytical interest. Therefore, the washing procedure used needs to be considered very carefully. Properties of washing procedures used before hair analysis were studied. The plan of experimental work was built up using of statistical methods of design of experiment. The effect of single useable cleaning media (nitric acid, acetone, Triton X-100, EDTA) was tested in a model based on a full factorial experiment, four factors at two levels. The optimization of cleaning conditions (temperature, time, and agitation support) was assessed using a full factorial experiment, three factors at three levels. 15 elements were followed (ICP-OES) including these which set up the border between exogenous (sodium, potassium) and endogenous (sulphur, copper) indicators. Significant differences between cleaning agents were found: each of them revealed different washing strength for specific elements. Surface impurities but also elements from the inner hair structure were removed (e.g. acetone released sulphur from the protein structure).	hair analysis; cleaning procedures;