Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Comparison of Liposome- and Octanol-water Distribution Constants of Antioxidants

Autoři: Wiedmer Susanne | Váňová Jana | Česla Petr

Rok: 2016

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Comparison of Liposome- and Octanol-water Distribution Constants of Antioxidants	Antioxidants are naturally occurring aromatic secondary plant metabolites which can help protect the body from harmful free radicals and can improve immune function of the organism. They are able to scavenge free radicals and inhibit oxidation processes. The antiatherosclerotic, antiinflammatory, anticancer, antibacterial, antiviral, and antitrombogenic effects of antioxidants have been studied. In vivo effects of these compounds are dependent on their lipophilicity and hydrophilicity, which govern membrane and protein interactions. The lipophilicity can be expressed as the partition coefficient between polar and nonpolar phases. The hydrophobicity of analytes is commonly described with the octanol-water partition coefficient, however, in that case the effect of charge of analytes is not considered. To get a better understanding of the distribution of analytes between lipophilic and hydrophilic phases liposome-water partitioning coefficients, which are dependent on van der Waals and hydrogen donor/acceptor interactions between analytes and the membrane, can be determined. In this work liposome electrokinetic chromatography (LEKC) was used for the study of interactions between natural antioxidants, specifically phenolic acids and flavonoids, and liposome dispersions. Liposomes comprising mixtures of 1-palmitoyl-2-oleyl-sn-glycerophosphatidylcholine with 1-palmitoyl-2-oleoyl-sn-phosphatidylserine or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyl-DL-glycerol were used as the pseudostationary phases in LEKC. The retention factors were determined at pH 7.4 and the phase ratios were calculated from the composition of the lipid mixtures. The liposome-water distribution constants were determined and the values were compared with literature values of octanol-water distribution constants for the studied antioxidants.