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Lipidomic analysis: comparison of supercritical fluid chromatography, liquid chromatography and direct-infusion mass spectrometry for analysis of lipids

Autoři: Lísa Miroslav | Cífková Eva | Ovčačíková Magdaléna | Holčapek Michal

Rok: 2016

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	Lipidomic analysis: comparison of supercritical fluid chromatography, liquid chromatography and direct-infusion mass spectrometry for analysis of lipids	Lipidomics is a branch of metabolomics aimed at the full analysis of lipid species and their biological roles in the organism. Lipids play multiple roles in cellular functions. They form membrane bilayers, participate in the cell growth, multiplication, death and signaling processes. Due to their important functions in living organisms, they are considered as possible biomarkers of some serious human diseases, such as cancer, cardiovascular diseases, diabetes, etc. Lipidomic analysis is a challenging task for analytical chemists due to the extreme complexity of lipidomic samples given by numerous lipid species widely differing in their structures, polarities and concentrations. The aim of this work is a comparison of chromatography/mass spectrometry (MS) and direct-infusion MS based approaches for the lipidomic analysis, which are compared in one laboratory using identical analytical protocol and the same instruments. For this purpose, ultrahigh-performance supercritical fluid chromatography/electrospray ionization-MS (UHPSFC/ESI-MS) method based on 1.7 μm particle-bridged ethylene hybrid silica column and methanol/water/ammonium acetate modifier is used for the lipidomic analysis. Hydrophilic interaction liquid chromatography - ultrahigh-performance liquid chromatography (HILIC-UHPLC) is applied for the lipidomic analysis using the identical type of column and mass spectrometer enabling the direct comparison of results with UHPSFC method. Lipid class separation of lipids according to polarity is achieved by both methods with the identification of fatty acyl composition based on positive and negative-ion full-scan and tandem mass spectra measured with high mass accuracy and high resolving power. UHPSFC/ESI-MS method provides the separation of both nonpolar and polar lipids in one 6 minute analysis, while nonpolar lipids are not separated using HILIC-UHPLC/ESI-MS.	lipidomic, LC, MS, SFC

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