Publikace detail

Retention behavior of lipids in reversed-phase ultrahigh-performance liquid chromatography coupled with electrospray ionization mass spectrometry

Autoři: Ovčačíková Magdaléna | Lísa Miroslav | Cífková Eva | Holčapek Michal

Rok: 2016

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Retention behavior of lipids in reversed-phase ultrahigh-performance liquid chromatography coupled with electrospray ionization mass spectrometry	Lipids fulfill multiple essential roles within all eukaryotic cells in living organisms [1]. The dysregulation of the lipid metabolism contributes to numerous serious human diseases, such as obesity, diabetes, cardiovascular diseases and cancer. Therefore, they are investigated as possible biomarkers of these diseases [2]. Lipidomics is a systematic study which includes detailed identification of individual cellular lipid species. Reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC) method using two C18 columns coupled in series is developed with the goal to separate and identify a high number of lipid species in biological samples. The identification is performed by the coupling with high-resolution tandem mass spectrometry (MS/MS) using quadrupole – time-of-flight (QTOF) instrument. Electrospray ionization (ESI) full scan and tandem mass spectra are measured in both polarity modes with the mass accuracy better than 5 ppm, which provides a high confidence of lipid identification. 416 lipid species covering 14 polar and nonpolar lipid classes from 5 lipid categories are identified in total lipid extracts of human plasma, human urine and porcine brain.

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