Publikace detail

HILIC-HPLC/ESI-MS analysis of gangliosides in biological samples

Autoři: Hájek Roman | Jirásko Robert | Lísa Miroslav | Cífková Eva | Holčapek Michal

Rok: 2016

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	HILIC-HPLC/ESI-MS analysis of gangliosides in biological samples	Gangliosides are a class of sphingolipids [1] characterized by ceramide lipid chain attached to the oligosaccharide moiety that varies in the complexity based on the level of sialylation (N˗acetylneuraminic acid, NeuAc) and uncharged sugars (GlcCer, Gal, GlcNAc, GalNAc, Fuc). These lipids are abundant in the central nervous system and play important roles in many physiological processes in cells, such as memory control, cell signaling, neuronal recovery, neuronal protection or apoptosis. Gangliosides are also related to some serious disease such as cancer, Alzheimer disease, Tay-Sachs disease and Fabry disease. Gangliosides are extracted using chloroform, methanol and water. Aqueous phase containing gangliosides is further purified on C18 solid-phase extraction column to remove undesired salts before LC˗MS. The main goal of this study is the development of analytical method for ganglioside analysis based on the hydrophilic interaction liquid chromatography coupled to negative-ion electrospray ionization mass spectrometry (HILIC/ESI-MS).

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