Publikace detail

HILIC-HPLC/ESI-MS analysis of gangliosides in biological samples

Autoři: Hájek Roman | Jirásko Robert | Lísa Miroslav | Cífková Eva | Holčapek Michal

Rok: 2016

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	HILIC-HPLC/ESI-MS analysis of gangliosides in biological samples	Gangliosides are a lipid class containing a backbone of sphingoid bases and polar saccharide chains with at least one sialic acid. These lipids are abundant in the central nervous system and play important roles in many physiological processes in cells, such as memory control, cell signaling, neuronal recovery, neuronal protection or apoptosis. Recently, gangliosides have also become an attractive target of biomarker discovery. Gangliosides are extracted using chloroform, methanol and water, where aqueous phase containing gangliosides is further purified on C18 solid-phase extraction column to remove undesired salts. The aim of this study is the development of a reliable analytical method for ganglioside analysis based on the hydrophilic interaction liquid chromatography coupled to negative-ion electrospray ionization mass spectrometry (HILIC/ESI-MS) applicable for complex biological samples. The optimization of chromatographic parameters including the buffer type, its concentration and pH value is performed to achieve the optimal HILIC separation with regard to the best sensitivity and chromatographic resolution of studied compounds.

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