Publikace detail

Analytical Monitoring of Lipidomic Changes for Selected Types of Cancer

Autoři: Holčapek Michal | Cífková Eva | Lísa Miroslav | Ovčačíková Magdaléna | Khalikova Maria

Rok: 2016

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Analytical Monitoring of Lipidomic Changes for Selected Types of Cancer	This research requires high-throughput, robust and validated mass spectrometry based methods for the quantitation of large number of lipids from multiple lipid categories and classes. Shotgun ESI-MS/MS is used for the direct quantitation of several phospholipid and sphingolipid classes in total lipid extracts without a chromatographic separation. Separation - MS approaches can increase the sensitivity due to the reduction of matrix effects and improve the confidence of the identification. Hydrophilic liquid chromatography (HILIC) or ultrahigh-performance supercritical fluid chromatography (UHPSFC) methods are convenient for the separation of lipid classes, where the coelution of class internal standards and analytes guarantee the same ionization efficiencies. Our UHPSFC method enables the quantitation of up to 30 lipid classes within 6 minutes, which is well suited for high-throughput analysis

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