Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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English

Publikace detail

Immobilized magnetic proteins and enzymes in bioanalysis

Autoři: Slováková Marcela

Rok: 2017

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Immobilized magnetic proteins and enzymes in bioanalysis	Magnetic micro and nanoparticles were used in biochemical and biological analysis as they offer the advantage of straightforward and fast handling by using magnets or magnetic coils (Figure 1). Immobilized biologically active molecules, i.e. enzyme, peptides, antibodies, removed by magnetic separation (Figure 2) can be gently and repeatedly used, which improves the economics of such processes while also making them more environmentally friendly. Magnetic particles development, connected to microfluidic devices has become a field of great interest. Microfluidic systems showed a potential for a high-throughput detection and characterisation of large sets of proteins necessary for proteomics. Covalent methods of protein immobilization bring advantageous attributes, i.e. long term ligand stability and biological activity. Specifically, immobilization of protease on magnetic support has in recent years been regarded as a potentially powerful procedure, because of the reduced autolysis products. Hence, several covalent immobilization methodologies will be presented. In some cases, oriented immobilization approaches, were used. Developed magnetic carriers with biologically active ligands were applied in bioanalysis and diagnosis; examples will be given, as proteolytic cleavage of protein substrates on-chip, in vitro protein phosphorylation, enzymatic glycoprotein modification, detection of specific antibodies in serum by magnetic ELISA.	Immobilized; magnetic; proteins; enzymes; bioanalysis