Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Proteomic investigation of biomarkers associated with remission of multiple myeloma disease

Autoři: Kročová Eliška | Jankovičová Barbora | Kupčík Rudolf | Brůčková Lenka | Maisnar Vladimír | Bílková Zuzana

Rok: 2017

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Proteomic investigation of biomarkers associated with remission of multiple myeloma disease	Cancer immunomics has become an intense area of research since 1960s, when Baldwin’s group demonstrated that solid tumors can be identified and destroyed by the host immune system. The increased incidence of autoantibodies in malignant tumors has been described since 1970s. These antibodies represent molecular signatures of anti-tumor immune response, thus being significant candidates for biomarkers. Moreover, due to presence of antibodies in sera, they can be screened easily using noninvasive approaches. Our work was focused on analysis of specific autoantibodies present in sera of patients with multiple myeloma in long-lasting remission, which could be a key factor documenting activity of immune system against specific tumor cells. For this purpose serological proteome analysis (SERPA) was used as powerful approach. The potential tumor associated antigens originating from different tumor cell lines (SKBR3 and HEL) were separated by two-dimensional gel electrophoresis (2-DE). Afterwards, the proteins were transferred onto nitrocellulose membrane by western-blot technique (WB). Membrane was incubated with sera of 7 patients or healthy individuals. Immunoreactive spot patterns were compared and cancer-associated proteins were in-gel digested and analyzed using MS and MS/MS (MALDI LTQ Orbitrap XL). In this pilot experiment, several interesting immunoreactive proteins were detected by 2-DE/WB with chemiluminescent detection and MS analysis, namely alpha enolase, glyceraldehyde-3-phosphate dehydrogenase and ATP synthase beta subunit. These proteins are known as differentially expressed proteins repeatedly identified in various pathophysiological processes. These results are presumed as a good starting point for complex clinical study to find out more information about immunoproteome of patients with multiple myeloma in long lasting remission.	immunoproteomics; multiple myeloma; SERPA