Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

DETERMINATION OF OXYLIPINS IN CLINICAL SAMPLES USING UHPLC/MS

Autoři: Chocholoušková Michaela | Jirásko Robert | Holčapek Michal

Rok: 2018

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	DETERMINATION OF OXYLIPINS IN CLINICAL SAMPLES USING UHPLC/MS	Oxylipins (eicosanoids, octadecanoids, docosanoids) formed from polyunsaturated fatty acids (PUFA), such as arachidonic acid (AA) and docosahexaenoic acid (DHA) are a special family of lipids. They can be generated by either enzymatic pathways using cyclooxygenase (COX), lipoxygenase (LOX), and cytochrome P450 (CYP450) or non-enzymatic oxidation. Oxylipins play an important role in physiological functions (e.g., cell proliferation, blood pressure, inflammation), and they are associated with diseases (e.g., cancer, diabetes, heart diseases). The most common approach for the analysis of oxylipins in human plasma samples is an ultrahigh-performance liquid chromatography coupling with mass spectrometry (UHPLC/MS) using selected reaction monitoring (SRM) and electrospray ionization in the negative-ion mode (carboxylic group in oxylipin structures). We developed the targeted UHPLC/MS method for determination of 63 oxylipins in human plasma samples, validated (calibration curve, limit of detection, limit of quantitation, matrix effect, recovery rate, precision, and accuracy) and applied to 40 clinical samples (20 breast cancer patient and 20 healthy volunteers). For quantitation, 14 deuterated internal standard were used. In human plasma samples, we detected 35 oxylipins and 20 of them quantified. Results were statistically evaluated using multivariate data analysis (MDA), such as principal component analysis (PCA), orthogonal partial least square-discriminant analysis (OPLS-DA), S-plot, and box-plots.	OXYLIPINS; UHPLC/MS