Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Determination of free polyphenols in buckwheat

Autoři: Pravcová Kateřina | Česlová Lenka

Rok: 2018

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Determination of free polyphenols in buckwheat	Buckwheat is a pseudocereal that is recognized as a nutritional food and medicinal plant all around the world. Many of the health benefits of buckwheat have been attributed to high levels of phenolic compounds and antioxidant activity in comparison to other cereals. The primary antioxidants in buckwheat are rutin, swertiamacroside, quercetin, orientin, vitexin and catechins. As well as being a major dietary source of rutin, buckwheat also contains other well-known antioxidants such as tocopherols and anthocyanins. The objective of this study was to analyse selected polyphenols in buckwheat using reverse-phase high performance liquid chromatography with tandem mass spectrometry (RP-HPLC/MS/MS). The extraction of free polyphenols from buckwheat flour was achieved using 70% acetone. The quantification of major free polyphenols in extracts was performed on octadecylsilica gel stationary phase using optimized gradient separation with aqueous methanol (acidified by formic acid) as a mobile phase. In addition, the spectrophotometric methods, total polyphenol content and antioxidant capacity were used to determine the total amount of polyphenolic compounds in the buckwheat extract. Optimized chromatographic and spectrophotometric methods were applied for analysis of several samples of buckwheat and the content of individual compounds was compared using different chemometric methods.	buckwheat; HPLC/MS; polyphenolic compounds