Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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English

Publikace detail

Characterization of cadmium induced apoptosis

Autoři: Čapek Jan | Brychtová Věra | Handl Jiří | Majtnerová Pavlína | Roušar Tomáš

Rok: 2018

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Characterization of cadmium induced apoptosis	Cadmium (Cd) is a heavy metal which is highly toxic. Cadmium has nephrotoxic and hepatotoxic effects linked with induction of oxidative stress. Acute cadmium exposure causes apoptotic cell death. Cd in cells activates pro-apoptotic factors and leads to the activation of caspases and DNA damage. In our study, we focused on comparison of methods for detection of apoptosis in cells treated with Cd. We used Human Kidney-2 cells (HK-2) treated with Cd at concentrations 25- 100 µM CdCl2 for time periods (6-24 h). In our work, we used various methods for detection of apoptosis such as: detection of mitochondrial membrane potential (JC-1 probe), DNA condensation (Hoechst 33258), detection of DNA fragmentation (TUNEL assay) and detection of caspase 3/7 activity (Caspase 3/7 kit). We observed the decrease of mitochondrial membrane potential in cells treated with 100 µM Cd after 24 h. We observed condensation of nucleus in both concentrations of Cd (25 and 100 µM) at both time duration (6 and 24 h). DNA fragmentation was observed in cells treated with 100 µM Cd after both time periods. Finally, caspase activity was increased at both tested Cd concentrations after 6 h. We conclude, all four methods detected apoptosis in Cd treated cells. After comparison of the methods, we found a strong correlation – the most sensitive apoptosis assay in Cd toxicity characterization was caspase 3/7 activity assay.	Cadmium; DNA damage; Kidney cells; Apoptosis