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Development of the magnetic carrier for the anti-beta amyloid 1-42 magnetic ELISA assay

Autoři: Slováková Marcela | Murasová Pavla | Vozandychová Věra | Bílková Zuzana

Rok: 2019

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	Development of the magnetic carrier for the anti-beta amyloid 1-42 magnetic ELISA assay	Objectives: An occurrence of the natural specific antibodies against the beta-Amyloid 1-42 in patient sera with neurodegenerative disorders is examined using the magnetic ELISA with a newly developed magnetic solid phase. Magnetic solid phase preparation comprised the beta-Amyloid 1-42 immobilization on superparamagnetic magnetic beads. A major effort was made to reduce the nonspecific sorption of serum contaminants to the prepared bioaffinity magnetic carriers. Further, experiments testing the in vitro cytotoxicity of selected magnetic beads show their safe disposal. Methods: Superparamagnetic beads ProMag carboxyl and Micromer Amine varying in composition and size were characterized by scanning electron microscopy, dynamic light scattering and zeta potential measurement. Activation of superparamagnetic beads was performed by carbodiimide chemistry followed by beta-Amyloid 1-42 peptide immobilization. Unoccupied functional groups of beads were blocked by various low molecular polyethylene glycol agents or BSA. In vitro cytotoxicity test (XTT) was applied to assay the cytotoxicity of the selected superparamagnetic beads. Results: The bioaffinity magnetic carriers using ProMag carboxyl and Micromer Amine carrying the beta-Amyloid 1-42 ligand were successfully prepared. Techniques affecting the performance the non-specific sorption of the beads were evaluated. Both magnetic carriers were applied for magnetic ELISA detection of anti-beta-Amyloid 1-42 natural antibodies in human healthy and patient sera with neurodegenerative disorders. Conclusions: Analysis of patient sera with neurodegenerative disorders confirmed applicability of the newly developed anti-beta-Amyloid 1-42 bioaffinity magnetic carriers in the magnetic ELISA assay. Selected superparamagnetic particles can be considered as non-toxic.	carrier; anti-beta amyloid 1-42; magnetic; ELISA;

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