Univerzita Pardubice Fakulta chemicko- technologická

Univerzita Pardubice

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Publikace detail

Determination of free polyphenols in buckwheat using RP-HPLC/MS/MS analysis

Autoři: Pravcová Kateřina | Česlová Lenka

Rok: 2019

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

Tituly:

Jazyk	Název	Abstrakt	Klíčová slova
eng	Determination of free polyphenols in buckwheat using RP-HPLC/MS/MS analysis	Buckwheat is a pseudo cereal that is introduced into the diet as an alternative crop of renewed interest due to its nutritive and health promoting value. It is widely used as a food and medicinal plant. In comparison to other cereals, it has been attributed to high levels of biologically active compounds with antioxidant activity, including phenolic compounds. Buckwheat’s beneficial effects are due to the broad spectrum of flavonoids which have health benefits including reduction of cholesterol, inhibition of tumors, regulation of hypertension, as well as control of inflammation, carcinogenesis and diabetes. The major antioxidants in buckwheat are rutin, swertiamacroside, quercetin, orientin, vitexin, catechins and their derivatives. The objective of this study was to analyse selected polyphenols in buckwheat using reverse-phase high performance liquid chromatography with tandem mass spectrometry (RP-HPLC/MS/MS). Further, the total polyphenol content and antioxidant capacity were determined using spectrophotometric methods. The extraction of free polyphenols from buckwheat flour was performed using 70% acetone. The separation of major free polyphenols in extracts was performed on octadecylsilica gel stationary phase using optimized gradient separation. Mobile phase was consisted of water acidified with 0.3% formic acid (A) and methanol (B). The quantification of selected phenolic compounds was performed using electrospray ionization mass spectrometry in multiple reaction monitoring mode. The influence of germination on the content of free phenolic compounds in buckwheat was investigated during this study. The change in the composition of the phenolic compounds were monitored using HPLC/MS/MS technique and also using spectrophotometric methods.	buckwheat, HPLC, mass spectrometry