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UHPSFC/MS: A powerful Tool for the Lipid Analysis of Biological samples

Autoři: Wolrab Denise | Chocholoušková Michaela | Jirásko Robert | Peterka Ondřej | Holčapek Michal

Rok: 2019

Druh publikace: ostatní - přednáška nebo poster

Strana od-do: nestránkováno

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Jazyk	Název	Abstrakt	Klíčová slova
eng	UHPSFC/MS: A powerful Tool for the Lipid Analysis of Biological samples	Lipids are important biomolecules with multiple biological functions. They are involved in energy storage, function as signaling molecules, and represent constituents of cell membranes. A dysregulation is associated to serious diseases, e.g., various types of cancer. The quantitation of lipids in biological samples may allow the differentiation of healthy and diseased donors. However, the analysis is challenging due to the structural and chemical diversity and the concentration differences of various lipid classes and lipid species in biological samples. Furthermore, lipid profiles may significantly differ between individual people due to exogenous factors, such as dietary intake, leading to high biological variability. Therefore, a highly robust, accurate and fast methodology is needed for the determination of lipid profiles for a statistical relevant number of biological samples, which should allow the differentiation of samples derived from healthy and diseased donors. The main aims were the validation, the UHPSFC/MS lipid profiling, the comparison of different mass spectrometry based methods, and finally the establishment of a quality control system for the analysis of plasma and serum samples obtained from healthy volunteers, and donors suffering from cancer. UHPSFC/MS measurements were carried out on an Acquity Ultra Performance Convergence Chromatography (UPC2) system coupled to a hybrid quadrupole traveling wave ion mobility time of flight mass spectrometer Synapt G2 Si from Waters (Milford, USA). Lipid classes were separated by employing an Acquity BEH UPC2 column in the gradient mode. Positive-ion ESI mode was employed for the analysis of lipid species belonging to glycerophospholipids, sphingolipids, and glycerolipids. Results show that UHPSFC/MS is a highly robust, precise, and a fast method for the analysis of polar and non-polar lipids. Over 100 lipids were quantified by using appropriate internal standards and isotopic correction in serum and plasma o	UHPSFC/MS; lipid analysis; biological samples

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